Primer

Part:BBa_K5406005:Design

Designed by: Efstathions Alonaris, Anna Nikoleta Griva, Vassili N. Kouvelis, Kristian Verdhi   Group: iGEM24_Athens   (2024-09-21)


Reverse Primer for GshFss Sequencing


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For a set of compatible primers to be designed, an alteration had to be made to the primer sequence, which now contains one base that is not contained in the original gene. That is base number 8 of the primer (A); in the orginial gene sequence, a G should have been in that place. In addition, the help of professor Vassili N. Kouvelis needs to be aknowleged.

Source

This part was made partially using Primer BLAST, a tool of the National Center for Biotechnology Information (NCBI) of the United States of America. The sequence for this part was made using as a template the GShFss gene as figured in part BBa_K5406000.

References